Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Mar 15;518:241–252. doi: 10.1016/j.virol.2018.03.006

Fig. 4. Persistent transcription from HIV uDNA in primary macrophages and microglia.

Fig. 4

(A) Monocyte-derived macrophages were infected with an equal p24 level of HIV-NL(KFS) or its non-integrating mutant HIV-vNL(KFS)(D116N) for 5 and 19 days. Cells were lysed and total cellular DNA and RNA were extracted and used for the quantification of viral DNA and Nef mRNA by real-time PCR and real-time reverse transcriptase PCR. (B) The relative ratio of Nef mRNA and HIV DNA was plotted. (C) Primary human microglia were infected with the non-integrating HIV-NL(KFS)(D116N) for 3 days. Cells were lysed and total cellular DNA and RNA were extracted and used for the quantification of viral DNA and Nef mRNA by real-time PCR and real-time reverse transcriptase PCR.