Table 1.
Number of unique HLA-I peptides identified.
| Workflow 1 | TREG | TCONV |
|---|---|---|
| Sample | CD4+/CD25+ | CD4+/CD25- |
|
| ||
| Donor #1 | 25 (0)† | NA |
| Pool 1 (4 donors) | 122 (1) | 1221 (1) |
| Pool 2 (8 donors) | 259 (0) | 3337 (2) |
|
| ||
| Total Peptides | 373 (1) | 4048 (3) |
| Workflow 2 | TREG | TCONV |
|---|---|---|
| Sample | CD4+/CD127low/CD25+ | CD4+ /CD25- |
|
| ||
| Donor #2 | 1163 (1) | 1758 (7) |
| Donor #3 | 223 (0) | 50 (3) |
| Donor #4 | 416 (2) | 1168 (9) |
| Donor #5 | 43 (0) | 179 (1) |
|
| ||
| Total Peptides | 1676 (3) | 2988 (19) |
| Activated / Expanded | ||
|---|---|---|
|
| ||
| Sample | Post activation | |
| Donor #5 | 2428 (10) | |
| Donor #6 | 5648 (44) | |
|
| ||
| Total Peptides | 7767 (52) | |
†
Number of identified HLA-I phosphopeptides listed in parentheses.