Figure 2. Reversal of Th2 Cytokine skewing during peanut desensitization.

A, Gating strategy for analysis of Treg and Teff cells. Violet^lowCD3⁺CD4⁺ T cells were further gated on CD4⁺CD25⁺CD127^low (Treg cells) and CD4⁺CD25⁻CD127^high (Teff) then analyzed for FOXP3 expression. B, Representative FACS plots of IL-4 and IFN-γ expression in Violet^low (peanut-specific) CD4⁺ Teff cells. C–F, percentages (Fig 2, C and D) and absolute numbers (Fig 2, E and F) of IL-4 and IFN-γ expression in peanut-responsive (Violet^low) CD4⁺ Teff cells in all 10 patients responding to OIT treatment measured at weeks 0, 12, 24, 52 and 104. G, Representative FACS plots of IL-4 and IFN-γ expression in Violet^low CD3⁺CD4⁺FOXP3⁺ Treg cells. H–K, percentages (Fig 2, H and I) and absolute numbers (Fig 2, J and K) of IL-4 and IFN-γ expression in peanut-responsive (Violet^low) CD4⁺FOXP3⁺ Treg cells in the same subjects as panels C–F at weeks 0, 12, 24, 52, and 104. Results represent means ±SEM. *p<0.05, **p<0.01, ***<0.001, ****<0.0001 by one way ANOVA with post test analysis with comparisons made to week 0 values.