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. 2018 Jun 12;9(45):27835–27850. doi: 10.18632/oncotarget.25600

Figure 3. L-OHP and CPT-11 produce different cytotoxic effects.

Figure 3

Cells were treated with 5 μM L-OHP, 10 μM CPT-11 or DMSO (Ctrl). (A) MTT assay measures metabolic activity of cells after 48 hour treatments (n = 3). (B) Flow cytometric analysis of subG1 cells after 48 hours treatments (n = 3). (C) Immunostaining with Annexin V-FITC and flow cytometry detecting apoptotic cells after 48 hour treatments (* p < 0.05, **p < 0.01, ***p < 0.001, n = 3). (D) Western blot analysis of caspase activation using antibodies against full-length and cleaved caspase-3, as well as cleaved PARP1; vinculin serves as loading control.