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Five cutoffs of ΔCt values were applied to separate methylated and unmethylated groups; (A) cutoff ΔCt of 0, (B) cutoff ΔCt of 2, (C) cutoff ΔCt of 4, (D) cutoff ΔCt of 6, (E) cutoff ΔCt of 8. The smallest p-value was obtained at the cutoff off 2.
