Fig. 1.

PDMF suppresses cell proliferation of A549 human lung adenocarcinoma. a Effect of PDMF on the proliferation of A549 cells was assessed by BrdU incorporation assay. A549 cells were incubated with medium (none), 580 ng/ml doxorubicin, or 0 (vehicle), 3, 7.5, 15, 30, 45, 60 or 75 μg/ml of PDMF for 24 h. Representative data of six independent experiments are shown as mean ± SD of three replicates. b The cytotoxic effect of PDMF on A549 cells was determined by trypan blue dye exclusion assay. A549 cells were incubated for 24 h with medium (none), 580 ng/ml doxorubicin, or 0 (vehicle), 30, 45, 60 or 75 μg/ml PDMF. Data are represented as mean ± SD from six independent experiments. ^* P < 0.05 indicates significant differences compared with vehicle by ANOVA with post hoc Dunnett’s test