Fig. 7.

The effect of selective mPGES-1 inhibition on CAF migration. (a) Representative images of immunohistochemical staining of mPGES-1, PDGFRβ and the IL-1 receptor type I (IL-1RI) and mPGES-1 and EP4 in tumors from untreated xenografted mice 9 days post tumor take. Scale bars indicate 100 μm. (b) Schematic representation and quantification of fibroblast migration towards SK-N-AS cells in the presence of CIII, vehicle (DMSO) control or EP4 antagonist. PGE₂ production was induced in fibroblasts by adding IL-1β prior to migration. Data is normalized to migration of untreated control represented as mean ± SEM. P-values are calculated using unpaired t-test. Each data point represents an individual experiment.