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. 2018 Jun 15;9(46):28042–28068. doi: 10.18632/oncotarget.25556

Figure 2. Antiproliferative activity of Au(NHC) and Au(NHC)2 against A2780 human ovarian cancer cells.

Figure 2

(A) Inhibitory concentration IC50 (μM) values were obtained after 72 h of treatment according to the procedure described by Skehan et al. [43]. The IC50 value of cisplatin was also assessed. (B) Cell viability time course upon Au(NHC) and Au(NHC)2 treatment using MTT assay. Values were obtained by measuring the percentage of treated-A2780 viable cells relative to untreated controls after 12, 24, 28, 72 h of incubation with the 72 h-exposure IC50-dose. (C) Cell cycle distribution was analysed by flow cytometric analysis. Histograms reported the cell percentage in G1, S and G2 phase of cell cycle after 48 h of treatment with the 72 h-exposure IC50-dose of Au(NHC) and Au(NHC)2. Flow cytometric images are representative of three independent experiments. All the experiments were performed at least in triplicate and results are reported as mean ± SD. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 (**p<0.01, ***p<0.001).