Figure 9. Effects of carbenes on the level of selected glycolytic and mitochondrial enzymes in IGROV1 and SKOV3 cells.
Western blot analysis of the glycolytic enzymes GAPDH, ALDOA, TPI1 (A) and of the mitochondrial enzyme CS (B) in IGROV1 and in SKOV3 cells upon exposure to Au(NHC) and Au(NHC)2. Representative Immunoblots are shown together with the corresponding Coomassie-stained PVDF membranes. Histogram reports normalized mean relative-integrated-density ± SD values of the GAPDH, ALDOA, TPI1 and CS bands. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).