Figure 2. Map of interactions of mutant 1 (Pro98Tyr) within the VNAR V13-VEGF₁₆₅ complex.

Interactions between V13 (represented on the vertical axis) and its VEGF₁₆₅ receptor (represented of the horizontal axis) are shown for complexes with the parental (A) and mutated (B) species. The color scale is a function of the interaction energy: redder shades indicate more favorable interactions, while bluer tints are less favorable. (C) Representation of the interaction at position 98 of V13 and position 51 of VEGF₁₆₅ before (white cartoon) and after (VEGF₁₆₅ green cartoon and V13 cyan cartoon) mutation. Blue circles show the amino acids that increase the interaction between the proteins.