Figure 3. Interaction map for Mutant 2 (Arg97Ala) within the VNAR V13-VEGF₁₆₅ complex.

Interactions between V13 (vertical axis) and VEGF₁₆₅ (horizontal axis) are shown for the parental (A) and mutated (B) forms. The color scale is a function of the value of the interaction energy: redder shades indicate more favorable interactions, while bluer tints are less favorable. (C) Representation of the interaction at position 97 of V13 and position 60 of VEGF₁₆₅ before (white cartoon) and after ( V13 green cartoon and VEGF₁₆₅ cyan cartoon) mutation. The blue circles show the amino acids that decrease the interaction between the proteins.