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(A and B) Forty-eight hours after seeding, HUVECs were left untreated (-VEGF165), treated with 4 ng/mL VEGF165 (+VEGF165), or treated with 4 ng/mL VEGF165 and 100 μM suramin (+VEGF165+suramin). Network branching was recorded for 240 hours. (C-E) Forty-eight hours after seeding, cells were treated with 4 ng/mL VEGF165 and the indicated concentration of each test VNAR. Network branching was recorded for 240 hours.
