Table 2. Frequency of genetic amplification involving different chromosomal regions in the test (series 1) vs the validation series (series 2-8) of GBM patients analyzed.

Number of amplified regions	Chromosomal regions involved	Amplified GBM cases
		Series 1 n=45/80 (56%)	Series 2-8 n=111/267 (42%)
One chromosomal region amplified (n=100; 29%)	7p 12q 4q 1q 12p 7q 13q 6p 8q 15q 16q 17q	17 (21%) 3 (4%) 4 (5%) 2 (3%) (0) (0) (0) (0) (0) (0) 1(1.2%) (0)	46(17%) 10 (4%) 6 (2%) 1 (0.4%) 2 (0.8%) 2 (0.8%) 2 (0.8%) 1 (0.4%) 1 (0.4%) 1 (0.4%) (0) 1 (0.4%)
Subtotal		27 (34%)	73 (27%)
Two chromosomal regions amplified (n=39; 11%)	7p,12q 1q,7p 1q,4q 7p,7q 3q,7p 4q,7p 4q,12q 1q,5p 1p7q 2q,12q 4q,9p 5p,7p 5q,7p 7p,11p 7p,9p 7p,14q 7p, Xp 17q,20q 20p,20q	4 (5%) 2 (3%) (0) 1 (1.2%) (0) (0) 2 (3%) (0) 1 (1.2%) (0) (0) (0) (0) 1 (1.2%) (0) (0) (0) (0) (0)	4 (1%) 3 (1%) 3 (1%) 2 (0.8%) 2 (0.8%) 2 (0.8%) 2 (0.8%) 1 (0.4%) (0) 1 (0.4%) 1 (0.4%) 1 (0.4%) 1 (0.4%) (0) 1 (0.4%) 1 (0.4%) 1 (0.4%) 1 (0.4%) 1 (0.4%)
Subtotal		11 (14%)	28 (10%)
≥Three chromosomal regions amplified (n=17; 5%)	1p,1q,10q 1p,7p,12q 1p,12q,17q 1q,7p,12q 4p,4q,12p 4q,7p,12q 4q,7q,12p 4q7q12q 4q,7p,18q 5q,6q,7p 6p,7q,12q 7p,17p,17q 2p,7p,12p,12q 4q,7p,7q,15q 7p11p11q12q 7q,12q,17q,20q 1p,4p,7p,11q,14q	(0) (0) 1 (1.2%) 1 (1.2%) (0) 1 (1.2%) (0) 1 (1.2%) (0) 1 (1.2%) (0) 1 (1.2%) (0) (0) 1 (1.2%) (0) (0)	1 (0.4%) 1 (0.4%) (0) (0) 1 (0.4%) (0) 1 (0.4%) (0) 1 (0.4%) (0) 1 (0.4%) (0) 1 (0.4%) 1 (0.4%) (0) 1 (0.4%) 1 (0.4%)
Subtotal		7 (9%)	10 (4%)

Results expressed as number (percentage) of cases showing gene amplification/chromosome arm.