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. 2018 Jun 15;9(46):27985–27997. doi: 10.18632/oncotarget.25462

Figure 4. Nup153 controls eNOS and ERβ nuclear translocation.

Figure 4

(A-B) Representative confocal microscopy images of eNOS (A) or ERβ (B) nuclear localization (green) in PCa cells before and after Nup153 depletion, untreated or treated with E2 (10-7M, 3h45min and/or 24 hours). Cells silenced for Nup153 (oligos mix, 60nM) or treated with scramble control oligo were analysed at 48h post-transfection. T3 treatment (10-7M, 3h45min) served as control of ERβ estrogen response specificity. Nuclei were counterstained with DAPI (blue) and merged with the Nup153 ([QE5], Abcam; red) and eNOS (Type III, BD; green) or ERβ (Genetex #110607; green) signals. Efficiency of Nup153 interference is assessed also by western blot (right upper panel A). (C) Representative confocal microscopy images of GFP localization in PCa-GFP transfected cells before and after Nup153 depletion. Nuclei were counterstained with DAPI and merged with the two protein signals (GFP in green and Nup153 [QE5] in red). Scale bar 50 μm. (D) Representative confocal microscopy images of HDAC2 localization (green) in PCa cells before and after Nup153 depletion. Nuclei were counterstained with DAPI and merged with the HDAC2 signal (green). Scale bar 50 μm.