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. 2018 Mar 22;37(26):3485–3500. doi: 10.1038/s41388-018-0161-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, and provide a link to the Creative Commons license. You do not have permission under this license to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — CREPT promoted colon cancer cell growth and tumor metastasis. a Expression levels of CREPT were increased after transfection with CREPT plasmids in colon cancer cell lines DLD-1 and HCT116 by RT-PCR and western blotting. b Knockdown efficiency of CREPT in HT29 and SW480 cell was confirmed by RT-PCR and western blotting. c Ectopic expression of CREPT significantly enhanced cell viability in both cell lines by MTT assay. d The number of colonies increased when transfected with CREPT plasmids in DLD-1 and HCT116. e Knockdown of CREPT significantly inhibited cell viability in HT29 and SW480 cell lines. f The number of colonies dramatically reduced after knockdown of CREPT in HT29 and SW480 cell lines. g Ectopic expression of CREPT significantly promoted cell viability in HCEC 1CT and 2CT cells. h CREPT overexpression accelerated HCT116 cells colony formation in soft agar. i Ectopic expression of CREPT in HCT116 cells promoted tumor growth and tumor weight in xenograft model. j Knockdown of CREPT in SW480 cells repressed tumor growth and tumor weight. IHC staining showed CREPT expression in xenograft tumor. n = 5. *P < 0.05, **P < 0.01