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. 2018 Jun 27;8:9731. doi: 10.1038/s41598-018-28157-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Remodeling of mitochondrial Ca²⁺ uptake and mitochondrial shapes in proliferative and quiescent GSLCs. (A) Representative PMT traces obtained from MitGA expressing TG1 cells in proliferating (black line, pH 7.4) or in quiescent (dotted grey line, pH 6.5) conditions. Values are plotted as L/L_TOTAL and every trace is the mean of 3 independent experiments. Prior to recording cells were washed with Ca²⁺-free medium, the arrows indicate the time at which medium containing 1 mM Ca²⁺ is perfused. (B) Confocal microscopy analysis of mitochondria shapes in proliferating (left panel, pH 7.4) and in quiescent TG1 cells (right panel, pH 6.5). Mitochondria were labeled with TOM20 antibody (green) and nucleus with Draq5 (red). Tubular mitochondria are found in proliferating TG1 cells (left panel and Inset) and donut-shaped mitochondria in quiescent TG1 cells (right panel and Inset). (C) Tubular (filled black bars) and donut-shaped mitochondria (striped black bars) quantified for the different culture conditions as indicated. Pictures were taken with a 63X 1.40 N.A. objective on a Leica SP8 upright confocal microscope. Scale bars: 10 µm in Insets; 5 µm for the other pictures.