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. 2018 Jun 27;8:9731. doi: 10.1038/s41598-018-28157-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial morphology remodeling in glioblastoma tumorospheres. (A) Confocal microscopy analysis of TG1 macro-tumorosphere section after 8 weeks in culture. Proliferating cells were labeled with the Ki67 antibody (red), mitochondria morphology revealed with the TOM20 antibody (green) and nucleus stained with Draq5 (blue). Arrow-heads point to typical tubular-shaped mitochondria and arrows to typical donut-shaped mitochondria. Insets are enlarged images of selected areas (dotted squares) in (a) and (b) domains of the macro-tumorosphere. Pictures taken with a 10 × 0.30 N.A. objective for whole views of tumorospheres and with a 63X 1.40 N.A. objective for (a) and (b) details on a Leica SP8 upright confocal microscope. Scale bars: 100 µm in all images in A and 5 µm in Aa and Ab. (B) Histogram plot of the number of cells with at least one tubular-shaped mitochondria or one donut-shaped mitochondria in the outer rim (a) and in the core (b) domains of the tumorosphere. 6 areas were counted in each domain.