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. 2018 Jun 27;9:2491. doi: 10.1038/s41467-018-04895-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Quantification of mtDNA rearrangements after DSB in the mutator mouse. a Diagram of wild-type C57BL6/J mtDNA illustrating the location and number of ScaI sites and primers used to quantify rearrangements following ScaI infection. Green primers amplify rearrangements that do not contain the origin of replication of the heavy-strand (O_H), purple primers amplify rearrangements that contain the O_H, orange primers amplify rearrangements that contain the O_H and the O_L. b Diagrams of putative C57BL6/J partially-deleted mtDNA after Ad-mitoScaI-HA infection and primers used to quantify rearrangements. One deleted molecule does not contain the O_H (green primers) whereas the other deleted molecule contains the O_H (purple primers). The last deleted molecule contains both the O_H and the O_L (orange primers). Quantification of the breakpoint without O_H in (c) mutator and wild-type fibroblasts at 1, 2, 5, and 10 days and (f) mutator and wild-type liver at 5 days after Ad-mitoScaI infection. Levels of the breakpoint without O_H were normalized to a region in the D-loop. Quantification of the breakpoint with O_H in (d) mutator and wild-type fibroblasts at 1, 2, 5, and 10 days and (g) mutator and wild-type liver at 5 days after Ad-mitoScaI-HA infection. Levels of the breakpoint with O_H were normalized to a region in the D-loop. Quantification of the breakpoint with O_H and O_L in (e) mutator and wild-type fibroblasts at 1, 2, 5, and 10 days and (h) mutator and wild-type liver at 5 days after Ad-mitoScaI-HA infection. Levels of the breakpoint with O_H and O_L were normalized to a region in the D-loop. NI indicates not infected. Quantification was performed using comparative ΔΔCt method. Error bars (s.e.m.) derive from technical replicates