Fig. 5.
Sequence analyses of mtDNA deletion breakpoints. PCR amplifications of mtDNA deletion breakpoints from DNA of fibroblasts and liver infected with Ad-mitoScaI-HA (wild-type and mutator) were cloned in a TOPO TA plasmid (TA cloning) and sequenced using M13F and M13R primers. a Five representative sequences from the deletions [with OH] and their frequency of detection in different samples. b Five representative sequences from the deletions [with OH and OL] and their frequency of detection in different samples. Breakpoints are annotated as follows, orange text - mtDNA that is retained, black text -mtDNA which is deleted, blue text – ScaI sequence, green text - size of the deletion breakpoint, underlined text - micro or imperfect homologies