Table 1.
Description of NAT gene constructs and their primate species of origin.
| Scientific name (synonym)a | Common namea | Taxon mnemonica | Taxon IDa | Geneb | GenBank IDb | Amplification primersc |
|---|---|---|---|---|---|---|
| Allenopithecus nigroviridis | Allen’s swamp monkey | ALLNI | 54135 | NAT1 | KU640972 | #1 and #2 |
| Cercopithecus diana | Diana monkey | CERDI | 36224 | NAT1 | KU640973 | #1 and #2 |
| NAT2 | KU640982 | #1 and #3 | ||||
| Chlorocebus tantalus (Cercopithecus tantalus) | Tantalus monkey | CHLTN | 60712 | NAT1 | KU640974 | #6 and #7 |
| NAT2 | KU640983 | #1 and #3 | ||||
| Erythrocebus patas (Cercopithecus patas) | Red guenon | ERYPA | 9538 | NAT1 | KU640975 | #6 and #7 |
| NAT2 | KU640984 | #1 and #3 | ||||
| Homo sapiens | Human | HUMAN | 9606 | NAT1 | X17059 (allele NAT1*4) | #1 and #2 |
| NAT2 | X14672 (allele NAT2*4) | Clone available | ||||
| Macaca mulatta | Rhesus macaque | MACMU | 9544 | NAT1 | KU640969 | #1 and #2 |
| NAT2 | AJ504440 (allele NAT2*1) | Clone available | ||||
| Macaca sylvanus | Barbary macaque | MACSY | 9546 | NAT1 | KU640970 | #1 and #2 |
| NAT2 | KU640978 | #1 and #3 | ||||
| Mandrillus sphinx (Papio sphinx) | Mandrill | MANSP | 9561 | NAT1 | KU640971 | #1 and #2 |
| NAT2 | KU640980 | #1 and #3 | ||||
| Nomascus gabriellae | Red-cheeked gibbon | NOMGA | 61852 | NAT1 | KU640967 | #1 and #2 |
| NAT2 | KU640985 | #1 and #4 | ||||
| Sapajus paella (Cebus apella) | Brown-capped capuchin | SAPAP | 9515 | NAT1 | KU640966 | #1 and #2 |
| Trachypithecus cristatus (Presbytis cristata) | Silvered leaf-monkey | TRACR | 122765 | NAT1 | KU640968 | #1 and #2 |
| NAT2 | KU640977 | #1 and #5 |
aThe current scientific names of species with synonymous names in parentheses (first column) and a common name (second column), are from the UniProt Taxonomy database (http://www.uniprot.org/taxonomy/). Official taxon mnemonics (third column) and taxon identification numbers (fourth column) are from the same source. According to current consensus nomenclature (http://nat.mbg.duth.gr/), taxon mnemonics are attached to the symbols of NAT genes to identify their specific organism of origin22.
bAll NAT gene annotations (fifth column) are as first described by6, except for the NAT2 gene of M. mulatta60, and the NAT1 and NAT2 genes of H. sapiens66. The recombinant proteins expressed in this study were as predicted by translation of the nucleotide sequences for which GenBank identification numbers are provided (sixth column).
cThe complete coding sequence of each NAT gene was amplified for cloning, using combinations of the following primers, as indicated (seventh column): Primer #1 (forward), GCGGCAGCCATATGGACATTGAAGCATA; Primer #2 (reverse), TCGAGTGCGGCCGCCTAAATAGTAAAAAATCTATCACC; Primer #3 (reverse), GTGCTCGAGTGCGGCCGCCTAAATAGTGAAGGATC; Primer #4 (reverse), TCGAGTGCGGCCGCCTAAATAGTAAGGGATCCATC; Primer #5 (reverse), TCGAGTGCGGCCGCCTAAATAGTAAAGAATCCATC. The recognition sites for restriction endonucleases NdeI (forward primer) and NotI (reverse primers) are underlined. Clones for the NAT2 genes of M. mulatta and H. sapiens were available in our laboratories from previous studies61. The NAT1 constructs of C. tantalus and E. patas were generated from the NAT1 construct of C. diana via site-directed mutagenesis with Primer #6 (forward), CCATGGACTTAGGCTTAGAGGCCAT, and Primer #7 (reverse), ATGGCCTCTAAGCCTAAGTCCATGG (in bold, the mutagenised nucleotide representing the only non-synonymous SNP differentiating the NAT1 gene of C. diana from the other two homologues). Amplification of the NAT2 genes of A. nigroviridis (GenBank ID: KU640981) and S. apella (GeneBank ID: KU640976) was not possible due to limited availability of genomic DNA.