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. 2018 Jun 27;9(7):728. doi: 10.1038/s41419-018-0757-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — M0, M1, and M2 macrophages were irradiated by different doses of protons. 2 h after the irradiation, the nuclear translocation of p65 was evaluated by NFκB p65 immunofluorescence labeling. NFκB p65 is stained in green and nucleus appears in blue. a Nuclear translocation of NFκB p65 is indicated (arrow) on representative immunofluorescence labeling images for M0, M1, and M2 macrophages after irradiation. b Quantification of the mean NFκB p65 intensity per nucleus 2 h after proton irradiation. Results are expressed in percentage of cells with nuclear NFκB p65. Quantifications were performed on minimum five images per condition (N = 3, mean ± SD). An unpaired t test was performed on data (*p ≤ 0.05; **p < 0.01; ***p < 0.001)