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. 2018 Jun 27;8:9732. doi: 10.1038/s41598-018-28095-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of the setup for automatic sleep detection and deprivation. Worms are kept inside microfluidic devices. The system allows continuous filming with DIC using transmitted infrared light and simultaneous fluorescence imaging. The DIC camera detects sleep using a frame subtraction algorithm using a dynamic threshold. We deprived the worm of sleep by dish tapping.