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. 2001 Oct 15;29(20):4114–4124. doi: 10.1093/nar/29.20.4114

Figure 2.

Figure 2

TSA stimulates transcription from the rRNA genes. Nuclei were isolated from NIH 3T3 cells treated for 24 h with the indicated concentrations of TSA and rDNA transcription was measured by nuclear run-on assay as described in Materials and Methods. In vitro labeled RNA was isolated using TRIzol reagent and purified by ethanol precipitation and/or Sephadex G-25 columns. The transcripts were hybridized to a fragment of the mouse rDNA promoter (–168 to +292) (10 µg DNA) immobilized on Zeta-Probe membrane. The results were visualized with a Molecular Dynamics PhosphorImager. (Upper) The results of a typical experiment. (Lower) The results from repeated experiments were quantitated and plotted relative to the control. Each point represents the mean ± SD (n = 7).