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. 2018 Jun 27;8:9747. doi: 10.1038/s41598-018-28114-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gp41-TCR interaction is not mediated by gp41 interactions with cholesterol. (A) Two-colour PALM imaging of fixed E6.1 Jurkat cells expressing gp41(JRFL)ΔED*-PAGFP and GPI-PAmCherry on coverslips coated with an αCD3 and αCD45. Cells were dropped and let spread on the coverslip for 3 min before fixation. Bars – 2 μm (left) and 200 nm (right). Shown is a representative cell (N = 16). (B) PCF of gp41-PAGFP (green) and GPI-PAmCherry (red). (C) EOM of gp41(JRFL)ΔED*-PAGFP and GPI-PAmCherry. (D) Two-colour PALM imaging of fixed E6.1 Jurkat cells expressing GPI-PAmCherry and gp41-mCRAC-Dronpa on coverslips coated with an αCD3 and αCD45. Cells were dropped and let spread on the coverslip for 3 min before fixation. (E) PCF of gp41(JRFL)ΔED*-mCRAC-Dronpa (green) and GPI-PAmCherry (red). (N = 6). (F) EOM of gp41(JRFL)ΔED*-mCRAC-Dronpa and GPI-PAmCherry. (G) Two-colour PALM imaging of fixed E6.1 Jurkat cells expressing gp41(JRFL)ΔED*-PAGFP and Gp41(JRFL)ΔED*-mCRAC-PAmCherry on an αCD3-coated coverslips. Cells were dropped and let spread on the coverslip for 3 min before fixation. (H) PCF of Gp41(JRFL)ΔED*-PAGFP (green) and gp41(JRFL)ΔED*-mCRAC-PAmCherry(red). (N = 7). (I) EOM of gp41(JRFL)ΔED*-PAGFP and gp41(JRFL)ΔED*-mCRAC-PAmCherry. (J) Two-colour PALM imaging of fixed E6.1 Jurkat cells expressing TCRζ-Dronpa and gp41(JRFL)ΔED*-mCRAC-PAmCherry on an αCD3-coated coverslips. Cells were dropped and let spread on the coverslip for 3 min before fixation. (K) PCF of TCRζ-Dronpa (green) and gp41(JRFL)ΔED*-mCRAC-PAmCherry(red). (N = 16). (L) EOM of TCRζ-Dronpa and gp41(JRFL)ΔED*-mCRAC-PAmCherry. Error bars are SEM.