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. 2018 Jun 27;8:9754. doi: 10.1038/s41598-018-28137-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ultrastructural aspects of the brain tissue of BALB/c mice infected with DENV-2. (A) Pyramidal neurons, (C) microglial cells and (D) astrocyte of non-infected mice showing regular aspects. (B) Pyramidal neuron exhibiting increased nucleus, swollen mitochondria and irregular nuclear membrane. (D) Microglial cell with increased nucleus and swollen mitochondria. (F) Astrocyte with heterochromatin deposition. Samples from infected mice were considered on the 7^th d.p.i. Het - heterochromatin; (Red asterisk) - mitochondria; MF - myelin fibers; N - nucleus; NM - nuclear membrane; d.p.i. - days post infection.