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. 2018 Jun 27;8:9768. doi: 10.1038/s41598-018-28083-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CQ reduces MMPs activity in vitro. (A) Representative images of gelatin zymography showing a CQ-driven decrease of MMP-9 and MMP-2 activity. Note that a CQ concentration as low as 10 µM has the same effect of TPEN (100 µM), here employed as a control. (B,C) Bar graphs show the quantification of images in A for MMP-9 and MMP-2, respectively (n = 6 per condition). Data are presented as mean ± SEM. “**” indicates p < 0.01.