Table 2.

Overview of anaerobic high-throughput experiments for determination of kinetic properties of C. kluyveri.

Exp ID	Experiment description#	EtOH	AA	BA	HA	Donor:Acceptor~
		mM	mM	mM	mM	—
A	Low AA	343*	0–257	<1	<0.1	1.3–∞
B	High AA	343	0–750	<4	<4	0.5–∞
C	EtOH inhibition	168 – 1764	60	<2	<10	0.1–30.6
D	BA	343	0	0–113	0	3.0–∞
E	BA with 1.6 mM AA	343	1.6	0.4–128	<1	2.8–212.4
F	BA with 6 mM AA	343	6	0–160	<1	2.8–51
G	HA inhibition	343	80	<3	5–184	3.8–4.6
H	Product formation – high AA	343	80	1.8	7.6	4.2
I	Product formation – low AA	343	34	1.7	7.4	9.6

Bold text indicates substrate or product concentration varied in experiments A–G (cf. experiment description).

^#Experiment description indicates which substrate or product concentration was targeted or what the goal of the experiment was.

^*All concentrations are actual initial concentrations as determined from the balch tubes at the start of the experiment.

^~Donor:acceptor is the ratio of electron donor (EtOH) to electron acceptor (AA + BA) at the start of the experiment.