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. 2018 Jun 27;8:9760. doi: 10.1038/s41598-018-28014-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal assessment of 2-NBDG fluorescence decay. (a) 2-NBDG fluorescence intensity over a 2-hour time period after topical application on a normal and a DMBA-treated hamster cheek pouch mucosa. Only 10 min, 90 min and 120 min time points are shown for clarity; (b) white light image and post 2-NBDG images of a non-necrotic tumor from the ROI (yellow outline) shown in (a); (c) change in fluorescence intensity over time with a 5 minute interval for ROIs selected from normal, OED and OSCC. Error bars represent five samplings within each site; (d) rate of 2-NBDG fluorescence intensity decay measured as the slope between 10 min – 120 min; (e) average variance in slope for normal, OED and OSCC with five samplings within each site. P-value * < 0.05, ** < 0.01.