Fig. 5.

Classification of epithelial cell classes furthest apart in the endometrial crypt base by synchrotron radiation spectral analysis using PCA-LDA, SPA-LDA and GA-LDA on FPA-FTIR microspectroscopy-derived data. a Micrograph with circles representing the regions sampled (this is a representation as the areas analysed were selected at different magnifications to reveal the synchrotron point spectral area). b Pre-processed spectra of the classes furthest apart in crypt bases. c Cost/function plot identifying the optimal number of PCs to be used for PCA. d Scores plot graphically representing classification by PCA-LDA. The x-axis represents the sample index and the y-axis DF1. e Wavenumber selection for SPA-LDA. f Scores plot graphically representing classification by SPA-LDA. The x-axis represents the sample index and the y-axis DF1. g Wavenumber selection for GA-LDA. h Scores plot graphically representing classification by GA-LDA. The x-axis represents the sample index and the y-axis DF1