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. 2018 Jun 27;35(9):169. doi: 10.1007/s11095-018-2448-8

Fig. 3.

Fig. 3

Neutrophils are crucial for FIR in HFCGR2–3 mice (a), Change of body temperature upon injection of 20 mg/kg HamTfR into HFCGR2–3 mice depleted of neutrophils (filled squares, ΔPMN) or left un-depleted (filled triangles). Un-depleted HFCGR2–3 mice injected with buffer instead of HamTfR served as control (open circles). (b), Depletion of neutrophils using mAb NIMP-R14 was confirmed by FACS analysis of peripheral blood of treated and control mice using FITC-labeled Gr-1 and APC-Cy7A labeled Ly6G mAbs. The neutrophil population is indicated by the square. (c), Serum levels of KC and MIP-2 of HFCGR2–3 mice depleted of neutrophils (ΔPMN) or left un-depleted after challenge with 20 mg/kg HamTfR. (d), Induction of ROS upon infusion of 20 mg/kg HamTfR mAb in HFCGR2–3 mice depleted (right panel) or non-depleted (left panel) of neutrophils (e), Change of body temperature of wild type mice adoptively transferred with purified human neutrophils and challenged with 20 mg/kg HamTfR (filled squares) or buffer control (filled triangles). Non-transferred mice challenged with 20 mg/kg HamTfR served as control (open circles).