Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 21;9:1327. doi: 10.3389/fmicb.2018.01327

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Wang, Yuan, Chen, Zheng, Xu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Apoptosis was detected by flow cytometry. MDRV p10.8 protein-induced cell apoptosis was investigated in DF1 cells treated with (a) mock, (b) plasmid pCI-neo-flg, (c) recombinant plasmid pCI-neo-flg-p10.8, (d) 2 μg/mL TM, (e) pCI-neo-flg-p10.8+TM, (f) 2 μg/mL TUDCA, (g) pCI-neo-flg-p10.8+TUDCA, (h) siPERK, (i) pCI-neo-flg-p10.8+siPERK, (j) sieIF2α, (k) pCI-neo-flg-p10.8+sieIF2α, respectively. After 24 h, cells were stained with Annexin V-FITC/PI and the proportion of apoptotic cells in each group were detected by flow cytometry. (A) Images of apoptosis were determined via flow cytometry in DF1 cells. Statistical analysis of the proportions of cell apoptosis in (B) p10.8, (C) TM and pCI-neo-flg-p10.8+TM, (D) TUDCA and pCI-neo-flg-p10.8+TUDCA, (E) siPERK and pCI-neo-flg-p10.8+siPERK, (F) sieIF2α and pCI-neo-flg-p10.8+sieIF2α compared with mock and pCI-neo-flg. ^∗P < 0.05, ^∗∗P < 0.01, the same as in the following study.