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. 2018 Jun 21;9:1327. doi: 10.3389/fmicb.2018.01327

FIGURE 5.

FIGURE 5

Muscovy duck reovirus p10.8 protein induced apoptosis via the BiP/PERK/eIF2α/CHOP pathway. (A,B) Protein p10.8 expression, ER index protein (BiP, PERK, p-PERK, eIF2α, and p-eIF2α) expression, and apoptosis index protein (CHOP, Caspase12, cleaved-Caspase12, Caspase3, and cleaved-Caspase3) expression in three groups of DF1 cells (mock, pCI, and p10.8) were analyzed by Western blot; β-Actin was used as the reference gene (the same as in the following study). Expression levels were statistically analyzed (∗P < 0.05, ∗∗P < 0.01, the same as in the following study). (C,D) Cells were treated with or without Tunicamycin (TM, final concentration 1 mmol/L) after transfection with pCI-neo-flg-p10.8, mock, and eukaryotic expression plasmid transfection (pCI) as the control. At 24 h post-transfection, the proteins of p10.8, ER index proteins, and apoptosis index proteins were determined by Western blot in the five groups, and the gray values were measured and statistically analyzed. (E,F) Further cells were treated with or without TUDCA (final concentration 1 mmol/L) after transfection with pCI-neo-flg-p10.8, protein (p10.8, BiP, PERK, p-PERK, eIF2α, p-eIF2α, CHOP, Caspase12, cleaved-Caspase12, Caspase3, and cleaved-Caspase3) expression was analyzed by Western blot, and expression levels were analyzed. (G–J) siPERK and sieIF2α were used to knockdown PERK and eIF2α expression, the images of protein expression were represented, and the gray values were measured and statistically analyzed.