FIGURE 3.

Cannabigerol action in cells expressing CB₁R or CB₂R. HEK-293T cells were transfected with 0.75 μg cDNA for CB₁R (red line) or 1 μg cDNA for CB₂R (blue line). Dose–effect curves for cAMP production are expressed as % of levels obtained by 0.5 μM forskolin treatment (A). Dose-effect curves for ERK1/2 phosphorylation are expressed as % respect to basal levels (B). Dose-effect curves for β-arrestin recruitment (C) and label-free (D) assays are expressed, respectively, in mBRET units and pm. In β-arrestin-2 recruitment assays cells were transfected with 1 μg cDNA for β-arrestin-Rluc and either 0.75 μg cDNA for CB₁R-YFP or 1 μg cDNA for CB₂R-YFP. Data are the mean ± SEM of a representative experiment in triplicates (n = 6).