Figure 3.

The cardiac Fgfr4/calcineurin/nuclear factor of activated T cell signaling is activated in kl/kl mice. (A) Quantitative real-time PCR analysis reveals no significant differences in Fgfr1 mRNA expression in cardiac tissue from both mouse lines compared to their respective wild-type controls. (B) Fgfr4 mRNA expression is elevated in hearts from kl/kl mice as well as Hyp mice; however, the differences are statistically significant only for the kl/kl animals. (C) Representative immunoblot and quantification indicate an upregulation of cardiac calcineurin protein expression only for kl/kl mice (Gapdh served as loading control). (D) Representative immunoblot and quantification of Nfat activation show reduced Nfat phosphorylation in cardiac tissue from kl/kl mice. Values are presented as mean ± SEM; numbers in each bar graph represent the n-values used for the respective measurement; *P < 0.05, **P < 0.01.