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. 2018 Jun 21;9:333. doi: 10.3389/fendo.2018.00333

Figure 7.

Figure 7

Calcium and phosphate treatment induces cell growth and pro-hypertrophic gene expression in cultured neonatal rat ventricular myocytes (NRVM). (A,B) Immunofluorescence staining for sarcomeric α-actinin followed by quantification demonstrates enhanced cardiac myocyte cross-sectional area after treatment of NRVM with calcium, phosphate, and PTH, but not 1,25D (magnification, 40×; scale bar, 50 µm). (C) Calcium and phosphate stimulation significantly increases Fgf23 mRNA expression in NRVM. (D) Rcan1 mRNA levels are significantly enhanced by treatment of NRVM with phosphate. The mRNA levels of (E) bMHC, (F) BNP, and (G) ANP are significantly upregulated after stimulation of NRVM with calcium and phosphate. Values are presented as mean ± SEM; numbers in each bar graph represent the n-values used for the respective measurement; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Abbreviations: Ca, calcium; Pi, phosphate; PTH, parathyroid hormone; 1,25D, 1,25(OH)2D3.