Table 1.

The following oligonucleotides shown in 5′–3′orientation were used as primers for quantitative RT-PCR analyses.

Gene	Orientation	Primer sequence (5′–3′)
Mouse Fgf23		Mm_Fgf23_1_SG QuantiTect Primer Assay QT01066772 (Qiagen, Hilden, Germany)
Mouse Fgfr1	Forward	TGC CAG CTG CCA AGA CGG TG
	Reverse	AAG GAT GGG CCG GTG AGG GG
Mouse Fgfr4	Forward	GGC TAT GCT GTG GCC GCA CT
	Reverse	GGT CTG AGG GCA CCA CGC TC
Mouse Rcan1	Forward	CCC GTG AAA AAG CAG AAT GC
	Reverse	TCC TTG TCA TAT GTT CTG AAG AGG G
Mouse aMHC	Forward	ACT GTG GTG CCT CGT TCC
	Reverse	GCC TCT AGG CGT TCC TTC TC
Mouse bMHC	Forward	AGG CAA GGC AAA GAA AGG CTC ATC
	Reverse	GCG TGG AGC GCA AGT TTG TCA TAA
Mouse ANP	Forward	ATT GAC AGG ATT GGA GCC CAG AGT
	Reverse	GA CAC ACC ACA AGG GCT TAG GAT
Mouse BNP	Forward	CCA GAT GAT TCT GCT CCT GC
	Reverse	TGA ACT ATG TGC CAT CTT GG
Mouse Col1	Forward	CCG CTG GTC AAG ATG GTC
	Reverse	CCT CGC TCT CCA GCC TTT
Mouse Mmp2	Forward	AAC TAC GAT GAT GAC CGG AAG TG
	Reverse	TGG CAT GGC CGA ACT CA
Mouse Tgfb1	Forward	TTG CTT CAG CTC CAC AGA GA
	Reverse	TGG TTG TAG AGG GCA AGG AC
Mouse Klotho	Forward	TGG GAA GGT TTT GTC CAG AAG A
	Reverse	AGA AAC GAG ATG AAG ACC AGC A
Mouse Gapdh	Forward	TAT GTC GTG GAG TCT ACT GG
	Reverse	AGT GAT GGC ATG GAC TGT GG
Rat Fgf23	Forward	GCA ACA TTT TTG GAT CGT ATC A
	Reverse	GAT GCT TCG GTG ACA GGT AGA
Rat Rcan1	Forward	CTC ACA CAC GTG GAC CAC CA
	Reverse	CGC CCA ATC CAG ACA AAC AG
Rat bMHC	Forward	CTC CAG AAG AGA AGA ACT CC
	Reverse	CCA CCT GCT GGA CAT TCT GC
Rat ANP	Forward	AAA TCC CGT ATA CAG TGC GG
	Reverse	GGA GGC ATG ACC TCA TCT TC
Rat BNP	Forward	CCA GAA CAA TCC ACG ATG C
	Reverse	TCG AAG TCT CTC CTG GAT CC
Rat Gapdh	Forward	ACT CCA CGA CAT ACT CAG CAC
	Reverse	CAT CAA CGA CCC CTT CAT T