Table 2.
List of grape stem polyphenols identified in studied cultivars.
| Peak | RT (min) | Phenolic class | Compound assignment | m/z [M-H]- | Product ions (ES-) | m/z [M+H]+ | λmax (nm) | References |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.69 | Phenolic acid | Gallic acid | 169 | 163, 125 | 269 | Sun et al., 2007 | |
| 2 | 9.65 | Stilbenoid DP1 | E-resveratrol | 227 | 183, 143 | 229 | 305, 317 | Pawlus et al., 2012; Lambert et al., 2013 |
| 3 | 9.17 | Stilbenoid DP1 | E-piceatannol | 243 | 185, 159 | 245 | 322 | Pawlus et al., 2012; Lambert et al., 2013 |
| 4 | 5.38 | Flavonoid | Catechin | 289 | 261, 205 | 291 | 229, 278 | Lambert et al., 2013 |
| 5 | 6.73 | Flavonoid | Epicatechin | 289 | 211, 152 | 291 | 229, 278 | Lambert et al., 2013 |
| 6 | 4.12 | Flavonoid | Gallocatechin | 305 | 109 | 307 | 332, 369 | Ehrhardt et al., 2014 |
| 7 | 8.43 | Stilbenoid DP1 | E-piceid | 389 | 227 | 278.7 | Ehrhardt et al., 2014 | |
| 8 | 8.48 | Flavonoid | Epicatechin 3-O-gallate* | 441 | 289, 169 | 443 | 276.7 | Ehrhardt et al., 2014 |
| 9 | 9.09 | Flavonoid | Astilbin | 449 | 303, 285 | 231, 289.7 | Souquet et al., 2000 | |
| 10 | 10.22 | Stilbenoid DP2 | Z-resveratrol dimer1* | 453 | 277, 265 | 455 | 232sh, 279, 285 | Püssa et al., 2006 |
| 11 | 13.39 | Stilbenoid DP2 | Z-resveratrol dimer2* | 453 | 428 | 455 | 225, 282.7 | Püssa et al., 2006 |
| 12 | 13.73 | Stilbenoid DP2 | E-ε-viniferin | 453 | 411, 369 | 455 | 225sh, 323 | Pawlus et al., 2012; Lambert et al., 2013 |
| 13 | 14.81 | Stilbenoid DP2 | E-ω-viniferin* | 453 | 369, 263 | 455 | 225sh, 323 | Püssa et al., 2006 |
| 14 | 15.36 | Stilbenoid DP2 | E-δ-viniferin* | 453 | 227 | 455 | 225sh, 309 | Pezet et al., 2003; Mulinacci et al., 2010 |
| 15 | 8.93 | Stilbenoid DP2 | Ampelopsin A | 469 | 451, 316 | 471 | 281.7 | Lambert et al., 2013 |
| 16 | 10.84 | Stilbenoid DP2 | Scirpusin A1* | 469 | 451, 395 | 471 | 324.7 | Kong et al., 2010; Moss et al., 2013 |
| 17 | 12.39 | Stilbenoid DP2 | Scirpusin A2* | 469 | 379, 301 | 471 | 321.7 | Kong et al., 2010; Moss et al., 2013 |
| 18 | 7.47 | Stilbenoid DP2 | Restrytisol A* | 471 | 455, 377, 246 | 230, 276 | Cichewicz et al. 2000; Jean-Denis et al., 2006; Mulinacci et al., 2010 |
|
| 19 | 8.32 | Stilbenoid DP2 | Restrytisol B* | 471 | 455, 379, 349 | 235, 267 | Cichewicz et al., 2000; Jean-Denis et al., 2006; Mulinacci et al., 2010 |
|
| 20 | 8.42 | Stilbenoid DP2 | Restrytisol 3* | 471 | 455, 389, 227 | 234, 330 | Cichewicz et al., 2000; Jean-Denis et al., 2006; Mulinacci et al., 2010 |
|
| 21 | 8.75 | Flavonoid | Quercetin-3-O-glucuronide | 477 | 301, 151 | 479 | 256, 354 | Souquet et al., 2000; Ehrhardt et al., 2014 |
| 22 | 4.76 | Procyanidin | Procyanidin B1 | 577 | 295, 162 | 579 | 280, 313 | Ehrhardt et al., 2014 |
| 23 | 5.13 | Procyanidin | Procyanidin B3* | 577 | 295, 162 | 579 | 280, 313 | Ehrhardt et al., 2014 |
| 24 | 6.18 | Procyanidin | Procyanidin B4* | 577 | 514 | 579 | Ehrhardt et al., 2014 | |
| 25 | 7.02 | Procyanidin | Procyanidin B2 | 577 | 579 | Ehrhardt et al., 2014 | ||
| 26 | 7.42 | Procyanidin | Procyanidin B5* | 577 | 522, 471 | 579 | Ehrhardt et al., 2014 | |
| 27 | 10.62 | Stilbenoid DP2 | Resveratrol dimer glycoside* | 615 | 567, 537, 453 | 323 | Moss et al., 2013 | |
| 28 | 14.71 | Stilbenoid DP3 | α-viniferin* | 677 | 423 | 679 | 230sh, 285 | Mattivi et al., 2011 |
| 29 | 12.34 | Stilbenoid DP3 | Resveratrol trimer1* | 679 | 681 | Püssa et al., 2006; Moss et al., 2013 |
||
| 30 | 12.91 | Stilbenoid DP3 | Resveratrol trimer2* | 679 | 383 | Püssa et al., 2006; Moss et al., 2013 |
||
| 31 | 13.33 | Stilbenoid DP3 | Resveratrol trimer3* | 679 | Püssa et al., 2006; Moss et al., 2013 |
|||
| 32 | 14.06 | Stilbenoid DP3 | E-miyabenol C | 679 | 573, 345 | 681 | 284 | Lambert et al., 2013 |
| 33 | 15.31 | Stilbenoid DP3 | Resveratrol trimer4* | 679 | 653, 454 | 681 | 230sh, 322 | Püssa et al., 2006; Moss et al., 2013 |
| 34 | 5.63 | Procyanidin | Procyanidin trimer* | 865 | 664, 576 | 867 | 279 | Monagas et al., 2006 |
| 35 | 14.45 | Stilbenoid DP4 | Dehydrogenated resveratrol tetramer * | 904 | 679 | 295 | Ito et al., 1999 | |
| 36 | 12.17 | Stilbenoid DP4 | Hopeaphenol | 905 | 811, 717, 705, 451, 359 | 907 | 281.6 | Lambert et al., 2013 |
| 37 | 12.56 | Stilbenoid DP4 | Isohopeaphenol | 905 | 811, 717, 451, 359 | 907 | 283.9 | Lambert et al., 2013 |
| 38 | 13.06 | Stilbenoid DP4 | Resveratrol tetramer1* | 905 | 907 | 285 | Püssa et al., 2006; Flamini et al., 2013 |
|
| 39 | 13.56 | Stilbenoid DP4 | Resveratrol tetramer2* | 905 | 907 | 284, 331 | Püssa et al., 2006; Flamini et al., 2013 |
|
| 40 | 16.03 | Stilbenoid DP4 | Resveratrol tetramer3* | 905 | 573 | 907 | 323 | Püssa et al., 2006; Flamini et al., 2013 |
| 41 | 16.2 | Stilbenoid DP4 | E-vitisin B | 905 | 799, 359 | 907 | 322 | Pawlus et al., 2012; Lambert et al., 2013 |
| 42 | 12.15 | Stilbenoid DP4 | Viniferol E* | 923 | Fujii et al. (2005) |
RT, retention time
*
tentative assignments based on MS data, UV spectra, elution order available from literature.