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. 2018 Jun 21;9:765. doi: 10.3389/fphys.2018.00765

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Copyright © 2018 Li, Chen, Cui, Zhang, Weng, Yu, Chen, Chen, Su, Yu, Wu, Huang and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of ERK in AGE-induced angiogenesis. HUVECs were pretreated with 20 μmol/L PD98059 for 30 min and then incubated with 100 μg/mL AGEs for 24 h. CCK-8 was used to evaluate the proliferation of HUVECs (A), scratch wound healing (B) and transwell migration assay (C) were used to measure the migration of HUVECs. The tube length, numbers of branch points, and vessel area were observed in Matrigel medium (D). n ≥ 4 independent experiments. ^∗P < 0.05 versus control, #P < 0.05 versus AGEs.