FIGURE 7.

The Role of Src/ERK pathway in AGE-induced aortic ring sprouting. The aortic rings were pretreated with 15 μmol/L PP2 for 90 min or 20 μmol/L PD98059 for 30 min respectively and then incubated with 100 μg/mL AGEs for 6 days. Fresh medium with or without AGEs was reintroduced every 2 days. An estimation of the capillary was performed by counting the branches from the aortic explants. Graph B displayed the statistical results of angiogenesis in mouse aortic ring. (A,B). n = 3 independent experiments. ^∗P < 0.05 versus control, #P < 0.05 versus AGEs. Scale bar: 200 μm. The aortic rings were incubated with isolectin B4 (green) for 1 h to stain endothelial cells for the indication of sprouts of neovessels (red arrows) (C).