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. 2018 Jun 21;9:660. doi: 10.3389/fphar.2018.00660

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Copyright © 2018 Liu, Peng, Liu, Wu, Wang, Wang, Liu, Xia and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TWEAK enhances the expression of palladin in human dermal fibroblasts. Cells were cultured in vitro, followed by TWEAK stimulation. (A,B) The mRNA and protein expression levels of palladin were measured after 48-h stimulation. ^∗p < 0.05, compared with the 0 ng/ml group; Δp < 0.05, compared with the 10 ng/ml group; #p < 0.05, compared with the 50 ng/ml group. (C,D) The mRNA and protein expression levels of palladin were measured after 250 ng/ml TWEAK stimulation (0–48 h). ^∗p < 0.05, compared with the 0 h group; Δp < 0.05, compared with the 12 h group. (E,F) By immunofluorescence and flow cytometry, the palladin expression was detected in cells that received siRNA transfection plus TWEAK stimulation (250 ng/ml, 48 h). ^∗p < 0.05, compared with blank group; Δp < 0.05, compared with TWEAK alone group; #p < 0.05, compared with TWEAK + control siRNA group. n = 3. Representative images are shown. Bar = 5 μm.