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. 2018 Jun 21;8:207. doi: 10.3389/fcimb.2018.00207

Figure 5.

Figure 5

Temperature-dependent regulation of PVD synthesis involved in virulence of Pseudomonas plecoglossicida. (A) Presence of PVD in the supernatants of cultures under different temperatures. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (B) The distance of the advancing color-change front in the CAS-blue agar under different temperatures. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (C) qRT-PCR analysis of the expression of pvds1, pvds2, pvds3, pvds4, and pvds5 after stable gene silencing compared with the control. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (D) Presence of PVD in the supernatants of stable silenced strains compared with the control. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (E) The distance of the advancing color-change front in the CAS-blue agar of stable silenced strains compared with the control. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (F) Growth of wild-type and stable silenced strains in the absence of 2, 2′-Dipyridyl (n = 3). (G) Growth of wild-type and stable silenced strains in the presence of 2, 2′-Dipyridyl (n = 3). (H) Biofilm formation of wild-type and stable silenced strains in the presence or absence of 2, 2′-Dipyridyl. Data are presented as mean ± S.D. (n = 6). Means of treatments not sharing a common letter are significantly different at P < 0.05. (I) The cumulative survival of E. coioides injected with wild-type and pvds1-, pvds2-, pvds3-, pvds4-, and pvds5-RNAi strains during 10 days post-challenge.