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. 2018 Jun 21;8:207. doi: 10.3389/fcimb.2018.00207

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Copyright © 2018 Huang, Liu, Jiang, Zuo, Su, Zhao, Qin and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temperature-dependent regulation of T6SS involved in virulence of Pseudomonas plecoglossicida. (A) qRT-PCR analysis of the expression of hcp, dotU, and icmF after stable gene silencing compared with the control. Data are presented as mean ± S.D. (n = 3). Means of treatments not sharing a common letter are significantly different at P < 0.05. (B) Biofilm formation of wild-type and stable silenced strains. Data are presented as mean ± S.D. (n = 6). Means of treatments not sharing a common letter are significantly different at P < 0.05. (C) The cumulative survival of E. coioides injected with wild-type and hcp-, dotU-, and icmF-RNAi strains during 10 days post-challenge.