Figure 10.

Induction of the splenic DC maturation and promotion of Th1-type cells by in vivo injection of MAP1981c. C57BL/6 mice (5 mice per group) were injected IV with 5 mg/kg MAP1981c or PBS and were analyzed 24 h later. (A) Single cells prepared from splenocytes of mice were stained with the Live/Dead (L/D) kit, anti-CD11c, anti-CD8α, anti-CD80, anti-CD86, anti-MHC-I, and anti-MHC-II Abs. The graphs show the expression levels of CD80, CD86, MHC-I, and MHC-II in CD8α⁺CD11c⁺ and CD8α⁻CD11c⁺ cells. All bar graphs show the means ± SEM from two independent experiments. (B) Mice (5 mice per group) of each group were injected with MAP1981c (5 mg/kg) or PBS, and after 3 days, were injected with MAP1981c (5 mg/kg) or PBS again. Single cells prepared from splenocytes of mice were stimulated with anti-CD3/CD38 Abs in the presence and absence of GolgiPlug for 12 h, and then the cells were stained L/D kit, anti-CD3, anti-CD4, anti-IFN-γ, anti-IL-4, anti-T-bet, and anti-GATA-3 Abs and analyzed by flow cytometry. The graphs show the expression levels of IFN-γ, IL-4, GATA-3, and T-bet in CD3⁺CD4⁺ or CD3⁺CD8⁺ T cells. All bar graphs show the means ± SEM from two independent experiments. n.s, No significant effects, *p < 0.05, **p < 0.01, ***p < 0.001.