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. 2018 Jun 21;8:206. doi: 10.3389/fcimb.2018.00206

Figure 4.

Figure 4

Confirmation of no LPS contamination of the recombinant MAP1981c. (A) DCs were treated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) in presence and absence of PMB. (B) DCs were treated with MAP1981c, LPS, boiled MAP1981c, and boiled LPS. (C) DCs were treated with MAP1981c and LPS in presence and absence of PK. After 24 h treatment, the culture supernatants were analyzed for the amounts of TNF-α, IL-6, and IL-1β by using ELISA. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown. The value of n. s. was defined as having no significant effect. The value of ***p < 0.001 was considered to be statistically significant compared with MAP1981c-treated DCs or LPS-treated DCs.