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. 2018 Jun 21;8:206. doi: 10.3389/fcimb.2018.00206

Figure 7.

Figure 7

The activation of MAPK and NF-κB signal transduction induced by MAP1981c. DCs were stimulated with MAP1981c (5 μg/mL) for various time points (0, 15, 30, and 60 min) and then suspended in lysis buffer. Protein levels in cell lysate were evaluated by immunoblotting analysis. (A) Expressions of phospho-p38 (p-38), non-phospho-p38 (p38), p-ERK, ERK, p-JNK, and JNK in cell lysate. (B) Expressions of p-IkB-α, and IkB in cell lysate. β-actin; loading control for cytosolic fractions. (C) Expressions of NF-kB p65 in nuclear fractions. Lamin B; loading control for nuclear fractions. The images shown are representative of three independent experiments. (D) The relative band intensity of each protein is expressed as a percentage. The data are shown as means ± SD (n = 3 samples). ***p < 0.001 for the treatments compared with untreated DCs (0 min). (E) The effects of MAP1981c on the cellular localization of the p65 subunit of NF-κB in DCs. The images shown are representative of three independent experiments (scale bar = 5 μm).