Fig. 2. Anti-inflammatory cytokine production profile of DCs differentiated from bone marrow cells in the presence of dexamethasone. Control cells were left to differentiate in the absence of dexamethasone. The supernatants from cultures were harvested 24 hours after activation with lipopolysaccharide for TNF-α (A), IL-12p70 (B), IL-1β (C) and MCP-1 (D), and the measurement of IL-10 (E) was performed 48 hours after stimuli with LPS. Cytokines were quantified by sandwich ELISA. The data represented is the median with a range of 4 (TNF-α, IL-12p70 and IL-1β), 7 (MCP-1) and 3 (IL-10), independent of experiments. DC, dendritic cell; TNF, tumor necrosis factor; IL, interleukin; MCP-1, monocyte chemoattractant protein-1; LPS, lipopolysaccharide; ELISA, enzyme-linked immunosorbent assay. ^*P<0.05 (Mann-Whitney's U test).