Fig. 3. Evaluation of Th2 parameters by dexamethasone-induced tolerogenic DCs. Experimental protocol of TolDC therapy in a mouse model of allergy to B. tropicalis (A). Mice were pre-treated with 1 or 2 doses of TolDCs prior to sensitization with BtE. Mice were euthanized 24 hours after the last challenge with BtE. The cellularity in BALF from naïve or Blomia-challenged mice, treated with 1 (TolDCs 1×) or 2 (TolDCs 2×) doses of TolDCs was evaluated. Total cell number (B), number of eosinophils in 200 cells (C) and differential (D) in BALF. Values are expressed as mean±SEM of 5–8 mice per group, in 1 of the 5 experiments performed. Th2, T helper type 2; DC, dendritic cell; TolDC, tolerogenic dendritic cell; BtE, B. tropicalis extract; BALF, bronchoalveolar lavage fluid; SEM, standard error of the mean. ^*P<0.05; ^†P<0.01; ^‡P<0.001; and ^§P<0.05 compared to the naïve and TolDC 2x groups (B and C, Dunn's multiple comparison test; D, Newman-Keuls multiple comparison test).