Fig. 6. Mucus analysis of lungs from allergic and tolerized mice. Lung sections of (A) control and (B) allergic mice treated with vehicle, (C) tolerized with TolDCs 1× or (D) tolerized with TolDCs 2×. Narrow arrows indicate areas of alcian blue⁺ cells (original magnification ×400). Scale bar, 50 µm. (E) Quantification of mucus production on alcian blue-stained lung sections. The area of alcian blue staining was estimated by morphometric analysis. Data is expressed as means±SEM of 5–8 mice per group, in 1 of the 2 experiments performed. TolDC, tolerogenic dendritic cell; SEM, standard error of the mean. ^*P<0.001 compared to vehicle-treated mice.