FIG 3 .

ΔgerS, ΔcwlD, and ΔpdaA spores are defective in cortex degradation but not SleC cleavage. (A) Purified spores were suspended in BHIS, taurocholate was added at a final concentration of 1%, and the optical density (OD₆₀₀) of each sample was measured out to 3 h. Statistical analysis was completed using repeated-measures ANOVA and Tukey’s test. Averages of results from three independent experiments performed using two independent spore preparations are shown, and the error bars indicate the standard deviation for each time point measured. (B) Western blot analysis of purified spores of one representative replicate following taurocholate treatment.