Table 2. Effect of CCCP treatment of COCs on mitochondrial DNA copy number and nucleic DNA copy number in cumulus cells, oocytes, and spent culture medium.
Groups | Cumulus cells |
Oocyte |
Spent culture media (100 μl) |
|||||
---|---|---|---|---|---|---|---|---|
No. of examined | mtDNA | No. of examined | mtDNA | No. of examined | mtDNA | Nucleic DNA | Ratio of mt/nucleic | |
Control | 11 | 514.7 ± 22.2 | 58 | 170,334 ± 8,642.7 | 11 | 1482.9 ± 142.9 a | 90.3 ± 11.5 | 17.7 ± 1.4 a |
CCCP | 11 | 497.3 ± 12.1 | 58 | 152,143 ± 9,791.0 | 11 | 2190.5 ± 185.6 b | 84.9 ± 11.2 | 29.1 ± 3.5 b |
Data are represented as mean ± SEM value. a–b: P < 0.05. Mitochondrial DNA copy number (mtDNA) was determined by real-time PCR targeting mitochondrial short sequences.