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. 2018 Apr 3;64(3):261–266. doi: 10.1262/jrd.2018-012

Table 2. Effect of CCCP treatment of COCs on mitochondrial DNA copy number and nucleic DNA copy number in cumulus cells, oocytes, and spent culture medium.

Groups Cumulus cells
Oocyte
Spent culture media (100 μl)
No. of examined mtDNA No. of examined mtDNA No. of examined mtDNA Nucleic DNA Ratio of mt/nucleic
Control 11 514.7 ± 22.2 58 170,334 ± 8,642.7 11 1482.9 ± 142.9 a 90.3 ± 11.5 17.7 ± 1.4 a
CCCP 11 497.3 ± 12.1 58 152,143 ± 9,791.0 11 2190.5 ± 185.6 b 84.9 ± 11.2 29.1 ± 3.5 b

Data are represented as mean ± SEM value. a–b: P < 0.05. Mitochondrial DNA copy number (mtDNA) was determined by real-time PCR targeting mitochondrial short sequences.